Gusmer Wine Catalog 2023-24

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Liquid Ampoule Culture Media Wine Application Guide


Part Number


Incubation Time Typical Colony

pH at 25 °C

& Temperature


A low pH selective culture medium for the detection of spoilage microorganisms resistant to acetic acid.

Preservative Resistant Yeast Broth

24 - 72 hrs at 30 °C


3.6 ± 0.2


Detection of Brettanomyces . Bacteria and other yeasts are inhibited.

Brettanomyces Broth

5 - 7 days at 30 °C

Colonies appear small, white and creamy.

3.5 ± 0.2


Mold can appear non-pigmented to white, with various texture. Yeast appear as creamy, white larger colonies. Bacteria appear blue-green. Yeast are large green opaque colonies. Mold appears green and filamentous. Bacteria able to grow at this pH form smaller, clear to white colonies.

48 hrs - 5 days for yeast at 30 °C. 35 °C for bacteria.

Wallerstein Nutrient Broth

Used to detect yeast and mold.

5.5 ± 0.2


A low pH culture medium used to detect yeast and mold and other aciduric organisms.

m-Green/ Schaufus Pottinger

48 - 72 hr at 28 - 32 °C

4.6 ± 0.2


Used to detect and enumerate bacteria present in small numbers in a mixed flora sample. Cycloheximide inhibits the growth of most yeast and mold, allowing bacteria to grow.

Bacteria appear small with green-blue color. If cycloheximide-resistant yeast grow, they are creamy, green white.

Wallerstein Differential Broth

48 - 72 hrs at 30 - 35 °C

5.5 ± 0.2


Used to detect yeast and mold. Chloramphenicol is used to suppress background bacterial growth.

Yeast and Mold Selective Broth

Yeast appear as large green and opaque colonies. Mold is green and filamentous.

48 - 72 hrs at 28 - 32 °C

4.4 ± 0.2


Note: Ampoules must be stored at 2 - 8 °C

46 2023 - 2024 Gusmer Enterprises, Inc. Wine Products Catalog

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