View Table of Contents
Liquid Ampoule Culture Media Wine Application Guide
Name
Part Number
Description
Incubation Time Typical Colony
pH at 25 °C
& Temperature
Appearance
A low pH selective culture medium for the detection of spoilage microorganisms resistant to acetic acid.
Preservative Resistant Yeast Broth
24 - 72 hrs at 30 °C
Yellow
3.6 ± 0.2
MHA00PRY2
Detection of Brettanomyces . Bacteria and other yeasts are inhibited.
Brettanomyces Broth
5 - 7 days at 30 °C
Colonies appear small, white and creamy.
3.5 ± 0.2
MHA00BSM2
Mold can appear non-pigmented to white, with various texture. Yeast appear as creamy, white larger colonies. Bacteria appear blue-green. Yeast are large green opaque colonies. Mold appears green and filamentous. Bacteria able to grow at this pH form smaller, clear to white colonies.
48 hrs - 5 days for yeast at 30 °C. 35 °C for bacteria.
Wallerstein Nutrient Broth
Used to detect yeast and mold.
5.5 ± 0.2
MHA000P2N
A low pH culture medium used to detect yeast and mold and other aciduric organisms.
m-Green/ Schaufus Pottinger
48 - 72 hr at 28 - 32 °C
4.6 ± 0.2
MHA000P2M
Used to detect and enumerate bacteria present in small numbers in a mixed flora sample. Cycloheximide inhibits the growth of most yeast and mold, allowing bacteria to grow.
Bacteria appear small with green-blue color. If cycloheximide-resistant yeast grow, they are creamy, green white.
Wallerstein Differential Broth
48 - 72 hrs at 30 - 35 °C
5.5 ± 0.2
MHA000P2D
Used to detect yeast and mold. Chloramphenicol is used to suppress background bacterial growth.
Yeast and Mold Selective Broth
Yeast appear as large green and opaque colonies. Mold is green and filamentous.
48 - 72 hrs at 28 - 32 °C
4.4 ± 0.2
MHA00P2SM
Note: Ampoules must be stored at 2 - 8 °C
866.213.1131 | www.GusmerWine.com 47
Powered by FlippingBook