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MilliporeSigma Microbial Monitoring Tools (continued)
Liquid Ampoule Culture Media Wine Application Guide
Granulated (Dehydrated) Media Granulated media offers several key advantages over traditional dehydrated media including: • Each granule contains the exact ingredient proportions • No settling or
Classic Filtration Setups and Accessories MilliporeSigma offers a full line of all manifold types, reuseable glassware, including filtering flasks and filter holders, classic vacuum pumps and other accessories for every lab setup.
Name
Part Number
Description
Incubation Time Typical Colony
pH at 25 °C
& Temperature
Appearance
A low pH selective culture medium for the detection of spoilage microorganisms resistant to acetic acid.
Preservative Resistant Yeast Broth
24 - 72 hrs at 30 °C
Yellow
3.6 ± 0.2
MHA00PRY2
separation of media ingredients that can lead to inconsistent tests • Faster dissolution • No sticking to glassware that can cause media waste and inconsistent results
Detection of Brettanomyces . Bacteria and other yeasts are inhibited.
Brettanomyces Broth
5 - 7 days at 30 °C
Colonies appear small, white and creamy.
3.5 ± 0.2
MHA00BSM2
Mold can appear non-pigmented to white, with various texture. Yeast appear as creamy, white larger colonies. Bacteria appear blue-green. Yeast are large green opaque colonies. Mold appears green and filamentous. Bacteria able to grow at this pH form smaller, clear to white colonies.
• Easier to weigh • Reduced dust and inhaling of particles
48 hrs - 5 days for yeast at 30 °C. 35 °C for bacteria.
Wallerstein Nutrient Broth
Used to detect yeast and mold.
5.5 ± 0.2
MHA000P2N
MilliporeSigma specifies the highest performance criteria available on the market in its media quality certificates.
A low pH culture medium used to detect yeast and mold and other aciduric organisms.
Many other media formulations and versions available
m-Green/ Schaufus Pottinger
48 - 72 hr at 28 - 32 °C
4.6 ± 0.2
MHA000P2M
Liquid Media and Petri-Pad ® Petri Dishes
Ready to use 2 ml plastic ampoules of sterile liquid media save time and effort when you only have a few samples to culture. No need to prepare media, simply uncap the ampoule and squeeze the sterile media into a Petri-Pad Petri dish’s absorbent pad. Petri-Pad sterile 47 mm Petri dishes, with pre-loaded pads, come fully assembled and ready to use. The pads conveniently absorb
Used to detect and enumerate bacteria present in small numbers in a mixed flora sample. Cycloheximide inhibits the growth of most yeast and mold, allowing bacteria to grow.
Bacteria appear small with green-blue color. If cycloheximide-resistant yeast grow, they are creamy, green white.
Wallerstein Differential Broth
48 - 72 hrs at 30 - 35 °C
5.5 ± 0.2
MHA000P2D
Used to detect yeast and mold. Chloramphenicol is used to suppress background bacterial growth.
Yeast and Mold Selective Broth
Yeast appear as large green and opaque colonies. Mold is green and filamentous.
48 - 72 hrs at 28 - 32 °C
2 mL of liquid media to instantly prepare it to receive an EZ-Pak or S-Pak membrane filter for culturing.
4.4 ± 0.2
MHA00P2SM
MilliporeSigma Glass filter holder with clamp
Note: Ampoules must be stored at 2 - 8 °C
36 2026 - 2027 Gusmer Enterprises, Inc. Wine Products Catalog
37 Order Info: cs@gusmerenterprises.com | 866.213.1131 | www.GusmerWine.com
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