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IFF Danisco ® Distilling Enzymes (continued) Saccharification Enzymes
Saccharification - Unfermentable carbohydrates from starch are broken down to produce fermentable sugars.
Product
Description
Benefits
Glucoamylase
• Increase alcohol production • Good for high corn mashes • Limits re-polymerization of glucose units which can occur in full sacchari cation • Use in conjunction with Diazyme FA or Alphalase AFP to optimize processing
DIAZYME ® X4
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Use Rate (kg/MT of grist)
Use Rate (ppm of grist)
Optimal Temperature Range
Optimal pH Range
GM Derived No
56 - 76 °C (133 - 169 °F)
300 - 600
3.5 - 5.5
0.3 - 0.6
Application: Add at temperatures below 70 °C (158°F) at saccharification for complete saccharification or in the fermenter.
Glucoamylase & protease
• Improve yeast vitality for consistent fermentations • Increase fermentation rate • Increase alcohol yields • Recommended for Simultaneous Sacchari cation and Fermentation
DIAZYME ® SSF
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Use Rate (kg/MT of grist)
Use Rate (ppm of grist)
Optimal Temperature Range
Optimal pH Range
GM Derived No
56 - 76 °C (133 - 169 °F)
400 - 1600
3.5 - 5.5
0.4 - 1.6
Application: Add to cooling mash when temperature reaches 60 – 65°C (140 - 149°F) before pitching yeast.
Fungal α-amylase, glucoamylase, & protease
• Faster initial alcohol production with increased alcohol yields from better starch hydrolysis • Protease activity provides nutrition for yeast for fast and complete fermentation • Obtain more fermentable sugars during fermentation • Recommended for Simultaneous Sacchari cation and Fermentation
DIAZYME ® SSF2
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Use Rate (kg/MT of grist)
Use Rate (ppm of grist)
Optimal Temperature Range
Optimal pH Range
GM Derived Yes
53 - 74 °C (127 - 165 °F)
600 - 800
3.5 - 5.5
0.6 - 0.8
Application: Add to cooling mash when temperature reaches 60 – 65 °C (140 – 149 °F) before pitching yeast.
Fungal α-amylase
• Increase alcohol production • Use in conjunction with Diazyme® X4 or Diazyme® SSF to improve processing
DIAZYME ® FA
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Use Rate (kg/MT of grist)
Use Rate (ppm of grist)
Optimal Temperature Range
Optimal pH Range
GM Derived No
38 - 58 °C (100 - 136 °F)
400 - 1600
3.1 - 6.4
0.4 - 1.6
Application: Add just before or at the beginning of fermentation in conjunction with Diazyme ® X4 or Diazyme ® SSF.
Glucoamylase & pullulanase
• Obtain more fermentable carbohydrates from dextrins • Increase alcohol yields and fermentation rate • Less residual starch in distillers grain
DIAZYME ® 480
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Use Rate (kg/MT of grist)
Use Rate (ppm of grist)
Optimal Temperature Range
Optimal pH Range
GM Derived Yes
60 - 65 °C (140 - 149 °F)
800 - 1000
4.0 - 5.0
0.08 - 0.10
Application: For processes using Simultaneous Saccharification and Fermentation, add directly to fermenter.
For pre-saccharification, add to cooling mash or liquefied starch when temperature reaches 60 – 65 °C (140 - 149°F).
West Coast: 559.485.2692 / East Coast: 908.301.1811 www.gusmerdistilling.com 7
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